產品[
Hs 738.St/Int 人成纖維細胞
]資料
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產品名稱:
Hs 738.St/Int 人成纖維細胞
產品型號:
CRL-7869
產品廠商:
美國標準生物品收藏中心（ATCC）
產品文檔:
無相關文檔
簡單介紹
CRL-7869 Hs 738.St/Int 人成纖維細胞，原代細胞|細胞系|細胞株|腫瘤細胞；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
Hs 738.St/Int 人成纖維細胞
的詳細介紹
CRL-7869 Hs 738.St/Int 人成纖維細胞
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CRL-7869?
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Hs 738.St/Int
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)CRL-7869 Hs 738.St/Int 人成纖維細胞
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fibroblast
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Organ: mixed; stomach; intestine
Disease: normal
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
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modal number = 46; range = 44 to 92
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18 weeks gestation fetus
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CRL-7869 Hs 738.St/Int 人成纖維細胞male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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CRL-7869 Hs 738.St/Int 人成纖維細胞
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