EBTr (NBL-4) 牛胚氣管細胞
ATCC® Number:  CCL-44?      
Designations:  EBTr (NBL-4) 
Depositors:   AJ Kniazeff, WA Nelson-Rees, NB Darby 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Bos taurus (cow) 
Morphology: fibroblast

 
Source: Organ: trachea
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Virus Susceptibility: bovine viral diarrhea; infectious bovine rhinotracheitis; parainfluenzavirus 3; herpes simplex; reovirus 3; vesicular stomatitis (Ogden); vaccinia
Virus Resistance: human adenovirus 5; poliovirus 2; coxsackievirus A9, B5 
Reverse Transcript: negative 
Age:  embryo 
Gender:  male 
Comments: The cells are negative for bovine viral diarrhea virus (BVDV). [22883]
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
27802: Vassilev VB, et al. Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts. J. Virol. 71: 471-478, 1997. PubMed: 8985373 
 
 

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