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CRL-1732 FOX-NY 小鼠**瘤細(xì)胞

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產(chǎn)品名稱: CRL-1732 FOX-NY 小鼠**瘤細(xì)胞
產(chǎn)品型號: CRL-1732
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔


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CRL-1732 FOX-NY 小鼠**瘤細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞株|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件

CRL-1732 FOX-NY 小鼠**瘤細(xì)胞 的詳細(xì)介紹
CRL-1732 FOX-NY 小鼠**瘤細(xì)胞
ATCC® Number: CRL-1732™    Price: $438.00
Designations: FOX-NY
Depositors:  RT Taggart
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments: FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.g., 2,6-diaminopurine and 8-azaguanine) as a result of deficiencies in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and adenosine phosphoribosyl transferase (APRT).
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
If the fusion products are grown in the presence of aminopterin, adenine and thymidine (or azaserine and adenine).
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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