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NG108-15 [108CC15]
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Univ. Texas Southwestern Medical Cntr.
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Mus musculus (neuroblastoma); Rattus norvegicus (glioma) (mouse (neuroblastoma); rat (glioma))
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flat; round; 10 to 100 micrometers diameter
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Organ: brain
Disease: glioblastoma; neuroblastoma
Cell Type: somatic cell hybrid
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
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ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium without sodium pyruvate . To make the complete growth medium, add the following components to the base medium:
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0.1 mM hypoxanthine (final conc.)
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400 nM aminopterin (final conc.)
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0.016 mM thymidine (final conc.)
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10% fetal bovine serum (final conc.)
Temperature: 37.0°C
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Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
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recommended serum:ATCC 30-2020
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51492: Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829
51493: Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920
112482: Bernd Hamprecht, personal communication
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