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TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾,骨髓瘤細胞

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產品名稱: TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾,骨髓瘤細胞
產品型號: TIB-146
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


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TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾,骨髓瘤細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和Z優培養條件!


TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾,骨髓瘤細胞 的詳細介紹
TIB-146 RA3 3A1/6.1 大鼠X小鼠雜合脾,骨髓瘤細胞
ATCC® Number: TIB-146?    Price: $399.00
Designations: RA3-3A1/6.1
Depositors:  RL Coffman
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments: Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
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